Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based (quantitative),
Assay Platform:Microplate reader
Product details:
Resazurin Assay Kit ab129732 is a fluorometric/colorimetric assay used to measure the metabolic capacity of live cells.
The resazurin assay is commonly used to quantify the number of live cells in a sample, and to monitor cell viability / cytotoxicity. It is a fast, simple, accurate and homogeneous / no-wash high throughput assay that can be used to monitor cells for up to 24-48 hours.
The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. Resorufin has Ex/Em of 530-560 / 590 nm and absorbance at absorbance at 570nm.
The resazurin dye can be measured in fluorescence or absorbance mode. However fluorescence mode measurement offers greater assay linearity, reproducibility, robustness and sensitivity.
Resazurin assay protocol summary:
- add resazurin reagent to cells in growth media and incubate for 4 hr at 37°C
- analyze with microplate reader
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our curated range of easy-to-use, efficient and fast cell viability assays
This kit was previously called Mitochondrial Viability Stain. Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
