Description
Key facts
Host species:Rat,
Target species:Mouse,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:Biotin,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Monoclonal,
Clone number:eB144,
Isotype:IgG,
Specificity:VeriBlot for IP secondary antibodies are anti-mouse immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa). In general this interference tends to originate from secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself. VeriBlot for IP secondary antibodies only recognize native (non-reduced) antibodies and therefore the detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced.
Product details:
Number of blots:
At least 20 blots
(based on a 0.5 μg/ml dilution in 5 ml milk).
Important protocol notes:
1. The anti-mouse VeriBlot for IP secondary antibody (Biotin) detects mouse IgG antibodies (subtypes IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse VeriBlot for IP secondary antibody (Biotin) preferentially detects the non-reduced form of mouse IgG (subtypes: IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-deantured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
5. Anti-mouse IgG VeriBlot for IP secondary antibody (Biotin) (ab131367) is the biotinylated form of the VeriBlot for IP anti-mouse HRP (ab131368). It can be used as a secondary antibody in combination with avidin/streptavidin HRP (ab59653)
ab7403
Western blot and IP resources:
Western blot a beginner's guide
IP protocol
IP troubleshooting tips
Properties and Storage Information:
Form-Liquid, Storage buffer-Preservative: 0.09% Sodium azideConstituents: 0.88% Sodium chloride, 0.16% Sodium phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
