Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:= 0.12 ng/mL,
Range:0.313 - 20 ng/mL,
Assay time:3h,
Assay Platform:Microplate
Product details:
Abcam's Complement C3a des Arg Human
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.
The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.
Cross Reactivity
Compound
Cross Reactivity
Human Complement C3a des Arg
Human Complement C3
Human Complement C4a des Arg
Human Complement C4
Human Complement C5 des Arg
Human Complement C5
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement C3a des Arg also known as the C3a desArg fragment is a bioactive derivative of the complement component C3a. This peptide is generated through the enzymatic removal of the carboxy-terminal arginine from C3a produced by the action of carboxypeptidase N. Its molecular mass is approximately 9 kDa. It is widely expressed in circulating blood particularly during the process of immune response activation. As a functional part of the complement system C3a des Arg plays a significant role in modulating immune responses and inflammation.
Biological function summary
The proteolytic removal of the terminal arginine from C3a results in C3a desArg which reduces its ability to activate C3a receptors. Despite this reduction in receptor activity C3a desArg retains anti-inflammatory properties and interacts with other components within the immune system. Unlike C3a it does not serve as a potent anaphylatoxin. It is not typically part of a larger molecular complex but acts independently to exert its effects on the immune system including modulation of leukocyte activity and interaction with distinct receptors compared to C3a.
Pathways
Complement C3a des Arg functions within the complement cascade a central component of the innate immune system. It plays roles in both the classical and alternative complement pathways influencing the immune response through modulation of inflammation. It associates with other proteins like C5a in specific regulatory pathways. The transformation from C3a to C3a des Arg can also influence interactions within the coagulation system where the futhan collection kit is utilized for monitoring such protein interactions during experimental procedures.
Complement C3a des Arg has associations with inflammatory conditions such as rheumatoid arthritis and systemic lupus erythematosus. Its role in modulating inflammation suggests a potential connection to these diseases where excessive immune activation occurs. The protein also shows interactions with C3 a related complement protein which plays an important role in autoimmune pathologies. Understanding these associations helps in the development of therapeutic strategies using tools like the C3a ELISA kit and other diagnostic assays.
