Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue, Serum,
Assay type:Competitive,
Sensitivity:= 40 pg/mL,
Range:160 - 100000 pg/mL,
Assay time:3h,
Assay Platform:Microplate
Product details:
Abcam's Direct 8-iso-PGF
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF
in Biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF
alpha antigen and a rabbit polyclonal antibody specific to 8-iso-PGF
alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF
alpha captured in the plate.
The 8-epimer of Prostaglandin F
(8-iso-PGF
) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF
has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis and a mutagen in 3T3 cells and in vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator and is able to modify the fluidity and integrity of membranes. 8-isoPGF
has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF
typically require the alkaline hydrolysis of 8-iso-PGF
esters from tissues, followed by length procedures involving extractions, phase separations and thin layer chromatography.
Cross Reactivity
Compound
% Cross Reactivity
8-iso-PGF
6-keto-PGF
13,14-dihydro-15-keto-PGF
6,15-keto-13,14-dihydro-PGF
8-iso-PGE
<0.001
<0.001
<0.001
2-Arachidonoylglycerol
<0.001
Anandamide
<0.001
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
8-iso-PGF2α also known as 8-iso-PGF2a iso-8-PGF2 or PGF2 alpha is an isoprostane formed by the free-radical-catalyzed peroxidation of arachidonic acid. It has a mass of approximately 354 g/mol. This molecule is commonly found in cell membranes during oxidative stress. It exists in various tissues with higher expressions noted in the liver heart and brain. 8-iso-PGF2α can be detected using methods such as the Alpha ELISA or Iso Kit which are critical for measuring its levels in biological samples.
Biological function summary
8-iso-PGF2α functions as a marker of oxidative stress within the organism. It participates in cell signaling affecting vascular tone and platelet aggregation. It does not form part of a larger protein complex by itself but acts through receptor-mediated mechanisms mainly interacting with thromboxane receptors to exert its effects. This interaction leads to vasoconstriction and plays roles in various cell signaling pathways.
Pathways
8-iso-PGF2α is involved in the oxidative stress response and the arachidonic acid metabolism pathways. It closely relates to the cyclooxygenase (COX) pathway intersecting with proteins such as COX-2 and modulating inflammatory responses. This interaction can influence the production of other prostanoids which further integrate into the inflammatory response pathways.
8-iso-PGF2α serves as a biomarker for conditions where oxidative stress is increased such as atherosclerosis and Alzheimer's disease. It connects to diseases through its impact on oxidative stress markers and its influence on vascular health. The molecule's interaction with thromboxane receptors and COX-2 makes it relevant in the progression of these diseases highlighting its potential as a therapeutic target for modulating oxidative damage in related conditions.
