Description
Key facts
Detection method:Fluorescent,
Assay type:Enzyme activity,
Assay Platform:Microplate reader
Product details:
How the assay works
Dipeptidyl peptidase IV (DPP4) Inhibitor Screening Assay Kit uses the fluorogenic substrate, Gly-Pro-Aminomethylcoumarin (AMC), to measure DPP4 activity. Cleavage of the peptide bond by DPP releases the free AMC group, resulting in fluorescence that can be analyzed using an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.
Dipeptidyl peptidase IV (DPP4) Inhibitor Screening Assay Kit protocol Summary
1. Prepare cells in assay buffer.
2. Add enzyme.
3. Add the Fluorescent Substrate to the wells.
4. Incubate at 37°C for 30 minutes.
5. Monitor fluorescence intensity using an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.
How other researchers are using
Dipeptidyl peptidase IV (DPP4) Inhibitor Screening Assay kit has been used in a variety of sample type including:
- Porcine pancreas 1
- Rat Blood samples 2
References:
1 - Lim W t al. 2022; 2 - Akash M et al. 2023.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
Biological function summary
DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.
Pathways
DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.
DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.
