Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Cell Lysate,
Assay type:Enzyme activity,
Range:0.02 - 0.2 µmol/min/mL,
Assay Platform:Microplate reader
Product details:
Abcam's PAF Acetylhydrolase Assay Kit (ab133088) provides an accurate and convenient method for measurement of PAF acetylhydrolase (PAF-AH) activity (both cytosolic and extracellular). The assay uses 2-thio PAF which serves as a substrate for all PAF-AHs. Upon hydrolysis of the acetyl thioester bond at the
-2 position by PAF-AH, free thiols are detected using 5,5'-dithio-
-(2- nitrobenzoic acid).
Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). PAF-AHs are located intra- and extra-cellularly (for instance, in both cytosol and plasma) Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the
-2 position and is associated with lipoproteins.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
