Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Cell culture supernatant,
Assay type:Cell-based,
Assay Platform:Microplate reader
Product details:
Glycerol Assay Kit (Cell-Based) (ab133130) provides a convenient tool for studying triglyceride/fatty acid cycling and its regulation in adipocytes or hepatocytes. This kit will allow investigators to screen compounds involved in lipid storage and metabolism. Chloroquine is included in the kit as a positive control for screening compounds that induce lipid droplet accumulation and free glycerol release from hepatocytes.
Other Notes
In mammals, triglycerides are constantly synthesized from fatty acids and segregated into cytosolic lipid droplets, mainly in adipocytes, as the major energy storage depot. During fasting, triglycerides stored in adipose tissue and liver are hydrolyzed by hormone-sensitive lipase and adipose triglyceride lipase to produce free fatty acids and glycerol. Triglyceride/fatty acid cycling is important in metabolic regulation and heat production, and is highly regulated by enzymes such as phosphenolpyruvate carboxykinase (PEPCK) and lipases. Quantitative changes in the triglyceride/fatty acid cycle have been related to the increased metabolic rate of cachectic patients with esophageal cancer and to metabolic syndrome. Abnormal triglyceride accumulation in the form of lipid droplets can occur in adipocytes and/or hepatocytes of obese mammals. In vitro, dramatic lipid accumulation can be observed in well-differentiated 3T3-L1 cells, or HepG2 cells treated with steatosis-inducing compounds such as chloroquine. Triglycerides stored in these lipid droplets can be hydrolyzed into free fatty acids and glycerol which are subsequently released into the surrounding environment. The amount of glycerol released into the medium is proportional to the triglyceride/fatty acid cycling rate.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycerol also known as glycerol anhydrous or glycérol is a simple polyol compound with a molecular weight of approximately 92.09 g/mol. Glycerol is expressed widely in various tissues and plays a considerable role as a backbone in the structure of triglycerides and phospholipids thereby impacting lipid biology significantly. It acts mechanically in lipid metabolism by serving as a substrate for glycerolipid synthesis and provides energy through its conversion to glucose or dihydroxyacetone phosphate.
Biological function summary
Glycerol metabolism is significant in energy storage and lipid regulation. It does not function in isolation but is part of various lipid complexes that regulate lipid homeostasis. Through its involvement it aids in the formation of essential lipid components that are important for membrane structure and function. The ability of cells to store energy efficiently is largely dependent on glycerol's capability to integrate into and support these lipid structures.
Pathways
Glycerol participates in glycerolipid metabolism and gluconeogenesis. The glycolytic pathway is especially important as glycerol serves as a potential precursor for glucose production connecting it to energy supply chains within the organism. Proteins like glycerol kinase and glycerol-3-phosphate dehydrogenase play critical roles in these conversions and metabolic processes underlying how glycerol fits into broader biological functions.
Glycerol has connections to metabolic conditions such as glycerol kinase deficiency and nonalcoholic fatty liver disease. Its metabolism's rate and efficiency can influence lipid accumulation and related metabolic syndromes. The enzyme glycerol kinase which phosphorylates glycerol to form glycerol-3-phosphate associates directly with glycerol's relevance in these diseases. Understanding these connections may lead to insights into managing or alleviating related metabolic disease symptoms.
