Description
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P06956,
Animal free:No,
Carrier free:No,
Species:Bacteriophage P1,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.08% Potassium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Product details:
Applications:
1. Protein intracellular transduction for in vitro Cre-loxP recombination.
2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.
Functional Assay: Mix 1μg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture 0.25 x10
293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium, will achieve > 35% transduction efficiency. (Positive for GFP cells).
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
