Description
Product details:
HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.
Concentration:
HeLa Lysate - Vehicle Treated Control - 200 μg at 1 mg/mL
HeLa Lysate - Calyculin Treated - 200 μg at 1 mg/mL
The lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
