Description
Key facts
Applications:Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Target:Nuclear yellow (Hoechst S769121)See target data,
Form:LyophilizedSee storage information,
Storage buffer:Constituents: 100% Nuclear yellow
Product details:
The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria.
The long-wavelength tracer Nuclear yellow is often combined with the popular retrograde tracer True Blue for two-color neuronal mapping. In neuronal cells, Nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas True Blue is a UV light–excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both Nuclear yellow and True Blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.
Molecular Weight: 651.01
Appearance: Light yellow powder
Solvents: water or dimethylsulfoxide (DMSO)
SPECTRAL PROPERTIES:
Excitation:
355 nm
Emission:
495 nm*
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store in the dark
