Abcam, ab138912-40mL, Media Supplement A

Key facts
Form:LiquidSee storage information,
Storage buffer:Preservative: 1.4% Gentamicin Reagent Solution 50 mg/mLConstituents: 28.2% MEM Amino Acids, 50X, 14.1% Antibiotic Antimycotic, 100X, 14.1% HEPES Buffer 1M Solution, 14.1% MEM Vitamins, 100X, 14.1% Sodium Pyruvate, 100mM Solution, 14% MEM Nonessential Amino Acids, 100X

Product details:
ab138912 is a supplement for hybridoma growth medium.
For complete growth medium:
Add 40 ml of ab138912 Media Supplement A (containing antibiotic/antimycotic/Gentamicin) to 500 ml of RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, and 10% fetal bovine serum. Incubate at 37°C.
Propagation:
Cultures can be propagated by centrifugation with subsequent re-suspension in fresh culture medium.
Establish new cultures at 1 x 10
cells/ml and maintain between 1 x 10
and 1 x 10
cells/ml.
Medium renewal:
Every 2 to 3 days.
Thawing guidance:
Freezing at over -70°C may cause some components of the media supplement (vitamins for example) to precipitate out of solution when thawing. Should this occur, heat the supplement in a 37°C water bath until the precipitate disappears. Alternatively thaw at 37°C instead of at room temperature.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C