Description
Key facts
Detection method:Colorimetric,
Sample types:Purified protein, Suspension cells, Tissue Extracts, Adherent cells,
Assay type:Enzyme activity,
Assay Platform:Microplate reader
Product details:
Akt Kinase Activity Assay Kit (ab139436) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of Akt and for quantitating the activity of Akt in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for Akt and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of Akt activity in the solution phase. For the measurement of Akt in partially purified and purified enzyme preparations from any species.
The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity
2. Flexible - kinetic and end-point options available
3. Fast - results in < 4.5 hours
4. Efficient - only 30 μl diluted sample needed per well
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKT also known as protein kinase B (PKB) is a serine/threonine kinase with a molecular weight of approximately 56 kDa. It plays an important role in signaling pathways regulating cellular processes such as glucose uptake cell survival and growth. AKT is expressed in various tissues including muscle and liver and is often observed using techniques like Western blot. Its kinase activity involves phosphorylation which is essential for its full activation and subsequent biological functions.
Biological function summary
AKT influences cell proliferation and apoptosis. As part of a signaling complex AKT phosphorylation modulates several downstream targets important for cell cycle progression and survival. Its activation depends on upstream kinases like PI3K and PDK1 positioning AKT as a central node in cellular responses. By mediating these processes AKT directly impacts organismal growth and stress response mechanisms.
Pathways
AKT is central to the PI3K/AKT/mTOR and insulin signaling pathways both important for cellular metabolism and growth regulation. It interacts with proteins such as mTOR and GSK3 which are involved in cell growth and glycogen synthesis respectively. These interactions show AKT's pivotal role in maintaining cellular energy balance and metabolic processes.
AKT is frequently linked to cancer and type 2 diabetes. Its abnormal regulation contributes to oncogenesis through uncontrolled cell proliferation and survival. AKT is also associated with insulin resistance in diabetes where it affects glucose metabolism. Through these conditions proteins like PTEN and IRS1 interact with or regulate AKT highlighting its significance in disease states and its potential as a therapeutic target.
