Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Fluorescence microscope
Product details:
Cellular ROS/RNS Assay Kit (ab139473) is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three fluorescent dye reagents: NO Detection Reagent (Red), Oxidative Stress Detection Reagent (Green) for total ROS detection, and Superoxide Detection Reagent (Orange). It also includes positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite.
**Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
