Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay time:30m,
Assay Platform:Fluorescence microscope
Product details:
Nucleolar Staining Kit - Green Fluorescence | Cytopainter (ab139475) contains a proprietary dye suitable for live-cell staining of nucleoli. The dye allows examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.ab139475 is specifically designed for visualizing nucleoli in living cells. The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™ or Nuclear Red dye.A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.Review other dyes and kits for , or the
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
