Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Microplate reader, Fluor. microscope, Flow cyt.
Product details:
The Chromatin Condensation Assay Kit (ab139479) provides a convenient approach for analysis of late stage apoptosis by flow cytometry and microscopy, and it can also be used in microplate assays. The basis of the assay is that the compacted chromatin of apoptotic cells binds higher amounts of nuclear dye compared to the healthy cells. The kit is suitable for differentiating between healthy and apoptotic cells with condensed nuclei.
A control apoptosis inducing agent, Staurosporine, is provided for monitoring apoptotic changes in nuclear organization. Potential applications for live-cell studies include quickly testing cell cultures for overall health, as well as rapid screening for compounds that induce apoptosis and chromatin condensation.
The cell-permeable dye used in this application is an aromatic, planar cationic structure that inserts between stacked base pairs on the DNA duplex, providing an environmentally-dependent fluorescence enhancement of the dye molecules and large increases in fluorescence signal relative to the free dye in solution. Since the signal enhancement provides a proportional response, direct quantitation of DNA is possible. Further signal increase is observed upon DNA condensation during apoptosis. Considering the general mutagenic effect of nucleic acid-binding dyes, careful storage and handling of this dye is recommended.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
