Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Direct,
Assay time:10m,
Assay Platform:Flow cytometer, Fluorescence microscope
Product details:
How the assay works
Annexin V-FITC Apoptosis Staining / Detection kit uses FITC labeled protein Annexin V that binds to the phosphatidylserine molecules on the apoptotic cell membrane, which is normally located on the inner side of a healthy cell, allowing the identification and quantification of early apoptotic cells using flow cytometry or a fluorescent microscope.
The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
The Annexin V-FITC reagent contained in the kit is also available as Annexin V-FITC Apoptosis Detection Reagent (500X)
ab14082
Annexin V-FITC Apoptosis Staining / Detection Kit protocol summary:
- Collect cells
- Resuspend cells in binding buffer.
- Add Annexin V-FITC and Propidium Iodide and incubate.
- Analyze by flow cytometry to quantify the percentage of apoptotic cells or by fluorescent microscope
Related and recommended products
See other alternative kits to quantify apoptosis using Annexin V with different labels:
- Annexin V-Cy5 Apoptosis Staining / Detection Kit
ab14150
- Annexin V-PE Apoptosis Staining / Detection Kit (with dead cell stain)
ab273274
- Annexin V-iFluor 350 Apoptosis Staining / Detection Reagent
ab219903
- Annexin V-iFluor 488 Apoptosis Staining / Detection Reagent
ab219904
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
Biological function summary
Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.
Pathways
Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.
Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
