Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Direct,
Assay time:10m,
Assay Platform:Flow cytometer, Fluorescence microscope
Product details:
Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144) includes Annexin V-Cy3, SYTOX green dye, and binding buffer. It can be used in a 10 min, one-step staining procedure to detect apoptosis and necrosis. Analysis is by flow cytometry or fluorescence microscopy.
The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids.
After staining a cell population with annexin V-Cy3 and SYTOX Green dye in binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.
These populations can easily be distinguished by fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).
The Annexin V apoptosis assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. For more apoptosis assays, review the full set of , or the .
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
