Description
Key facts
Applications:Flow Cyt, Fluorescence MicroscopySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.4Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 1% BSA
Product details:
Annexin V-Cy5 is a bright fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. Cy5 fluorescent dye produces an intense signal in the far-red region of the spectrum and therefore it is very useful for multiple labeling of cells with green and red colored fluorescent probes. Cy5 yields fluorescence with a λmax emission of 670 nm. Visit our for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called 1013 Annexin V-Cy5 Reagent.
Annexin V/ANXA5-Cy5 Apoptosis Detection Reagent ab14147 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V/ANXA5-Cy5 reagent can be used with Annexin V/ANXA5 binding buffer
ab14084
(see protocol section for instructions). The Annexin V/ANXA5-Cy5 reagent is also available as part of Annexin V/ANXA5-Cy5 apoptosis kit
ab14150
For more apoptosis assays, review the full set of
Annexin V/ANXA5 assays
, or the
apoptosis assay and apoptosis marker guide
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C
