Description
Key facts
CAS number:674289-55-5,
Form:LiquidSee storage information,
Molecular weight:292.3 Da,
Molecular formula:C18H16N2O2,
PubChem:3476986,
Nature:Synthetic,
Biochemical name:Blebbistatin,
Biological description:Cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 μM). Inhibits actin-activated MgATPase activity and *in vitro* motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.,
Canonical smiles:CC1=CC2=C(C=C1)N=C3C(C2=O)(CCN3C4=CC=CC=C4)O,
InChi:InChI=1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3,
InChiKey:LZAXPYOBKSJSEX-UHFFFAOYSA-N,
IUPAC Name:3a-hydroxy-6-methyl-1-phenyl-2,3-dihydropyrrolo[2,3-b]quinolin-4-one
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myosin and its variants such as the non-muscle heavy chain myosin tau MYH6 non-muscle myosin IIA and Myh14 serve mechanical roles in cellular processes. These proteins are part of class II myosins commonly referred to as myosin II with each having a different function based on tissue distribution. The molecular mass of these myosins can vary exemplified by MYH6 which weighs approximately 223 kDa. Expression for these proteins varies: MYH6 is found in cardiac muscle while non-muscle myosin IIA and Myh14 are predominantly expressed in non-muscle cells. Myosin interacts with actin filaments to mediate cellular contractility and motility executing force generation through ATP hydrolysis.
Biological function summary
These myosins assume pivotal roles in maintaining cell structure and function. They contribute significantly to cellular adhesion migration and division by interfacing systematically within the cytoskeleton. In contractile cells myosin works as part of a motor protein complex yet in non-muscle cells its responsibilities extend to cell shape maintenance and cytokinesis. These proteins directly influence cellular signaling dynamics and mechanotransduction. The non-muscle myosin IIA for example is critical for maintaining epithelial cell tight junctions emphasizing the structural diversity and importance of myosins across cell types.
Pathways
Myosin and its variants participate prominently in the actin cytoskeleton signaling pathway. This pathway orchestrates cellular movement and structure important for proliferation and morphogenesis. Myosin's role involves coordinated function with proteins like actin itself tropomyosin and myosin light chains facilitating intracellular transport mechanisms. Additionally myosin II has interactions within the Rho GTPase signaling pathway contributing further to regulation of the actin cytoskeleton and influencing processes like cell migration and focal adhesion dynamics.
Dysfunctional myosin proteins can link to a variety of conditions. MYH6 mutations for instance associate with cardiomyopathies which affect heart muscle function and structure. Another significant disorder is cancer where abnormal myosin expression or mutation promotes tumor progression and metastasis. Connections with tau highlight risk factors in neurodegenerative diseases where tau protein aggregation can influence cellular systems. The myosin-related dysfunctions influence diseases by acting in concert with other proteins as seen with TDP1 in neurodegeneration or STK33 in certain cancers reflecting the pervasive impact of myosin functionality in health and disease.
