Description
Key facts
Sample types:Tissue sections
Product details:
The Congo Red Stain Kit / Amyloid Stain Kit is intended for use in the histological visualization of amyloid in tissue sections. Examination under a polarizing microscope results in green birefringence of amyloid.
Other products for staining tissue sections
Find more kits and reagents in the
special stains guide
, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide
Staining Interpretation
Amyloid
Red to Pink
Erythorcytes
Light Orange
Eosdinophil Granules
Orange to Red
Nuclei
Control Tissue
Freshly cut sections containing amyloid.
Cut sections 6-12 microns to show smaller amyloid deposits.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Amyloid refers to protein aggregates known for their beta-sheet structure. These aggregates accumulate extracellularly in tissues and organs. The term ‘amyloid’ encompasses a collection of proteins including amyloid beta and amyloid precursor protein which can weigh around 4 kDa to several hundred kDa depending on the type. Amyloid deposits occur in the brain and other organs. Detected through methods like Congo red staining these aggregates are important in diagnosing amyloidosis.
Biological function summary
The proteins that form amyloid aggregates affect cellular function by altering normal protein conformation leading to toxicity. Amyloid can form complexes with other proteins which impact cell membranes and disrupt cellular homeostasis. These alterations trigger inflammatory responses and cellular stress pathways further damaging affected tissues. Understanding amyloid proteins provides insight into their role in disease states.
Pathways
Amyloid proteins interplay with several signaling cascades notably the MAPK and NF-kB pathways. Amyloid precursor protein processed to form amyloid beta significantly influences these pathways mediating cell death and inflammation. Proteins such as tau also interact with amyloid-related pathways impacting cellular transport mechanisms. These interactions highlight amyloid's role in cell signaling and pathology.
Amyloid proteins are strongly associated with Alzheimer's disease and systemic amyloidosis. The accumulation of amyloid beta aggregates in the brain marks the pathology of Alzheimer's closely linked with tau protein hyperphosphorylation and neurofibrillary tangles. In systemic amyloidosis amyloid deposition occurs in various tissues causing organ dysfunction. The relation of target proteins like apolipoprotein E to amyloid further elucidates its implications in neurodegenerative and systemic conditions.
