Description
Product details:
Masson's Trichrome Stain Kit ab150686 for histological staining of collagen, collagen fibers, fibrin, muscles, and erythrocytes. Three stains are used in Masson's trichrome staining: Aniline blue, Biebrich scarlet-acid fuschin, and Weigert’s Hematoxylin. Aniline blue is provided in this kit; in some other protocols a green dye is used to stain collagen instead of Aniline blue.
The trichrome staining protocol used in this kit is designed for use on formalin-fixed, paraffin-embedded tissue sections. For frozen sections, it will require optimization by the user.
Masson's Trichrome staining protocol summary
Deparaffinize sections if necessary
Preheat Bouin's Fluid to 54-64°C
Incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
Rinse in water
Incubate slide in Weigert's Iron Hematoxylin for 5 min
Rinse in water
Incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
Rinse in water
Differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
Incubate slide in Aniline Blue solution for 5-10 min
Rinse in water
Incubate slide in acetic acid solution for 3-5 min
Dehydrate, clear and mount
Staining Interpretation
Collagen
Blue (Aniline Blue)
Muscle Fibers
Red (Biebrich scarlet-acid fuschin)
Nuclei
Dark Red to Black/Blue (Weigert’s Hematoxylin)
Control Tissue:
Lung, Uterus, Small Intestine, Stomach.
Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:
Picro Sirius Red Stain Kit (Connective Tissue Stain)
ab150681
Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain)
ab150683
Reticulum Stain Kit (Connective Tissue Stain)
ab150684
How other researchers use Masson's Trichrome Staining
Based on a brief survey of publications using Trichrome Stain Kit ab150686, Masson's Trichrome staining is most often used to stain for fibrosis and collagen levels in liver, heart, and lung. It is also often used with pancreas, bladder, kidney, skin, adipose tissue, and vascular tissue.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagenous connective tissue fibers often referred to simply as collagen fibers are essential structural components in various tissues including skin bone tendons and ligaments. These fibers with a molecular mass that can vary depending on the type of collagen provide tensile strength and support to tissues and organs. They are synthesized by fibroblasts and expressed abundantly in extracellular matrix environments. Collagenous fibers are identifiable using Masson's trichrome staining which helps differentiate these fibers in histological studies.
Biological function summary
Collagenous fibers contribute to the mechanical framework of connective tissues enabling them to resist stretching and deformation. They are part of larger complexes that include elastin and proteoglycans working together to maintain tissue integrity and function. These fibers interact with other cellular components through specific receptors like integrins which mediate signals for cellular processes including differentiation adhesion and migration.
Pathways
Collagenous connective tissue fibers play roles in wound healing and tissue remodeling. They participate in pathways involving matrix metalloproteinases (MMPs) which regulate the degradation of extracellular matrix components. The collagen synthesis and cross-linking process often links with proteins such as fibronectin and laminin which influence cell behavior and tissue repair mechanisms.
Defective or insufficient collagenous connective tissue fibers can lead to conditions like Ehlers-Danlos syndrome and osteogenesis imperfecta. Both disorders reflect abnormalities in collagen synthesis or structure resulting in increased tissue fragility and elasticity issues. Mutations in collagen-related genes often underlie these pathologies highlighting the importance of these fibers in maintaining structural and functional integrity of various tissues.
