Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 25 pg/mL,
Range:0.025 - 6 ng/mL,
Assay Platform:Microplate
Product details:
Abcam's beta IG H3 (TGFBI) Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an
in vitro
enzyme-linked immunosorbent assay designed for the quantitative measurement of Human beta IG H3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human beta IG H3 (TGFBI) coated on a 96-well plate. Standards and samples are pipetted into the wells and beta IG H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta IG H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta IG H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization maybe required with urine samples.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
