Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue, Suspension cells, Tissue Homogenate, Serum, Adherent cells,
Assay type:Enzyme activity,
Sensitivity:< 1 mU/well,
Range:2 - 10 nmol/well,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
How the assay works
In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.
Creatine kinase assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37°C
How other researchers are using
Creatine Kinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human embryonic cardiomyocyte cell line H9C2
Mouse and rat glomerular tissues
Mouse skeletal muscle samples
References:
1-Mao M et al. 2023
2-Del Nogal Avila M et al. 2022
3- Buchalter D et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
