Description
Key facts
Detection method:Fluorescent,
Sample types:Cell culture extracts, Tissue Extracts,
Reacts with:Human,
Assay type:Enzyme activity,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
SIRT6 Activity Assay Kit (Fluorometric) (ab156068) detects deacetylase activity of recombinant SIRT6.
Primarily, the SIRT6 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT6.
2. Detecting the effects of pharmacological agents on SIRT6.
Other Notes
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SIRT6 protein also known as Sirtuin 6 plays an important role in cellular regulation. It is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases. SIRT6 weighs approximately 39 kDa and can be found in the nucleus of many cell types. It acts mainly in regulating cellular homeostasis by removing acetyl groups from histone proteins which affects gene expression. Its expression levels vary across different tissues and it is known to be active in metabolically active organs such as the liver and brain.
Biological function summary
SIRT6 influences DNA repair metabolism and inflammation. It participates in maintaining genomic stability by promoting base excision repair a critical DNA repair process. Moreover SIRT6 contributes to glucose homeostasis by influencing gluconeogenesis and glycolysis. This protein is not known to be part of any larger protein complexes but it interacts individually with other proteins to exert its biological effects.
Pathways
SIRT6 plays a significant role in two key biological pathways: DNA damage response and metabolism regulation. In the DNA damage response pathway SIRT6 works with other proteins like PARP1 to facilitate DNA repair under stress conditions. In the regulation of metabolism SIRT6 interacts with transcription factors like HIF1α which influences the expression of genes involved in glycolytic metabolism and glucose homeostasis.
SIRT6 has associations with cancer and aging-related diseases. SIRT6 has a protective effect against oncogenesis by maintaining genomic stability and regulating metabolic pathways that cancer cells exploit. Additionally its role in aging is connected to its ability to prevent age-related genomic instability and metabolic decline. In cancer SIRT6 interacts with proteins such as c-Myc and p53 influencing cell growth and apoptosis pathways. This highlights its potential as a therapeutic target for both cancer and age-related conditions.
