Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell Lysate,
Assay type:Quantitative,
Sensitivity:< 2 nmol/well,
Assay Platform:Microplate reader
Product details:
Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect G3P less than 2 nmol/well and can be used for a variety of sample types.
Other Notes
This product was previously called K641 Glycerol-3-Phosphate (G3P) Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycerol-3-phosphate (G3P) also known as glycerol phosphate is a significant metabolite in the body. G3P mechanistically functions by acting as an intermediate in lipid metabolism and energy production. The enzyme glycerol-3-phosphate dehydrogenase (GPDH) converts glycerol to glycerol-3-phosphate during glycolysis and triglyceride synthesis. The mass of G3P in metabolic processes is not directly applicable because it is a small molecular metabolite rather than a macromolecule. G3P is mainly expressed in liver and muscle tissues where energy regulation and lipid biosynthesis are important.
Biological function summary
The conversion of glycerol to glycerol-3-phosphate is an important step in various cellular functions. G3P plays a central role in triglyceride and phospholipid biosynthesis. It serves as a building block for the glycerol backbone in lipid molecules. In cellular respiration G3P is part of the glycerol-3-phosphate shuttle a mechanism in mitochondria that assists in transferring reducing equivalents from cytosolic NADH into the mitochondrial matrix linking G3P with the aerobic respiration process. This shuttle is not a complex by itself but it involves interactions with other mitochondrial components.
Pathways
Glycerol-3-phosphate is deeply entrenched in lipid metabolism and gluconeogenesis. It is an important player in the triglyceride biosynthesis pathway where it is esterified to form phosphatidic acid with acyl-CoAs. This process links it with enzymes such as acyl-CoA synthetase and phosphatidate phosphatase. Additionally the glycerol-3-phosphate shuttle pathway connects G3P to oxidative phosphorylation linking it to enzymes like mitochondrial glycerol-3-phosphate dehydrogenase and ubiquinone which are essential for ATP production.
G3P is significantly involved in metabolic diseases such as obesity and diabetes. Dysregulation in the balance of glycerol to glycerol-3-phosphate conversion can lead to aberrant lipid accumulation contributing to obesity. Additionally issues with the glycerol-3-phosphate dehydrogenase enzyme are linked to insulin resistance a hallmark of type 2 diabetes. In these conditions G3P's role interfaces with other proteins like insulin receptor substrate proteins which are critical in maintaining normal insulin signaling and glucose homeostasis.
