Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based (quantitative),
Assay Platform:Flow cytometer
Product details:
Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric – Green Ex 405 nm) (ab176739) is used to evaluate the viability of mammalian cells by flow cytometry.
The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/Em =408/512 nm excited with a violet laser at Ex=405 nm.
**Related assays**Review the to learn about kits to perform a , and .
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
