Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Sensitivity:< 0.5 U/mL,
Range:2 - 128 U/mL,
Assay Platform:Microplate
Product details:
Abcam's anti-Thyroglobulin
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroglobulin in Human serum or plasma.
A 96-well plate has been precoated with Human thyroglobulin to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroglobulin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroglobulin sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Anti-Thyroglobulin antibodies target the protein thyroglobulin an important component in thyroid physiology. Known also as TG thyroglobulin is a large glycoprotein with a mass of approximately 660 kDa. It is primarily expressed in the thyroid gland specifically in the follicular cells where it functions as a precursor for thyroid hormones. Thyroglobulin serves as the backbone for the synthesis of thyroxine (T4) and triiodothyronine (T3) essential hormones for metabolic regulation.
Biological function summary
Thyroglobulin connects to thyroid hormone production and is stored within the thyroid follicles as a colloid. It acts as a substrate for the synthesis of T3 and T4 hormones after iodination. Colloid resorption releases thyroid hormones into circulation influencing multiple physiological processes such as growth and metabolism. Thyroglobulin does not operate as a part of a complex; it undergoes enzymatic modification individually to produce active hormones.
Pathways
Thyroglobulin plays a critical role in the thyroid hormone synthesis pathway. Iodine uptake by thyroid cells and coupling with thyroglobulin is central to this process. It interacts closely with proteins like thyroperoxidase which catalyzes iodine addition and the sodium/iodide symporter essential for iodine uptake into cells. The pathway highlights the regulated mechanism that maintains hormone levels for metabolic activities.
Autoantibodies targeting thyroglobulin link to thyroid-related conditions such as Hashimoto's thyroiditis and Graves' disease. These conditions arise when the immune system mistakenly targets thyroglobulin causing dysregulation or destruction of thyroid tissues. Thyroglobulin antibodies often appear alongside anti-thyroperoxidase antibodies suggesting a combined role in triggering immune-related thyroid dysfunctions. Early detection of anti-thyroglobulin antibodies can help diagnose and manage these conditions effectively.
