Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:> 0.09 mU/mL,
Range:1 - 400 mU/mL,
Assay Platform:Microplate
Product details:
Abcam's Chorionic Gonadotropin beta
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma.
A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta IgG antibodies. Samples, standards and the Chorionic Gonadotropin beta-HRP conjugate are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody and added Chorionic Gonadotropin beta-HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Chorionic Gonadotropin beta also known as β-hCG is a major subunit of the human chorionic gonadotropin hormone. It is a glycoprotein with a molecular mass of approximately 22 kD. β-hCG predominantly expresses in placental syncytiotrophoblast cells and appears in the blood and urine. This hormone plays an essential role in reproductive processes and researchers often measure it using β-hCG ELISA kits.
Biological function summary
The beta subunit promotes pregnancy maintenance by supporting corpus luteum function and embryo development. β-hCG exists as part of the larger hCG hormone complex which includes an alpha subunit that it shares with other glycoproteins like luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Its binding to LH/CG receptors in the ovary makes it pivotal for progesterone secretion in early pregnancy.
Pathways
Chorionic Gonadotropin beta engages in reproductive hormone pathways significantly impacting the gonadotropin-releasing hormone (GnRH) signaling pathway. It relates to LH directly and its actions are indispensable in stimulating ovarian androgen secretion. These pathways further link it to progesterone production emphasizing its importance in fertility and pregnancy.
Chorionic Gonadotropin beta has notable connections with gestational trophoblastic diseases including molar pregnancy and choriocarcinoma. Elevated levels of β-hCG also indicate potential testicular cancers in males. Researchers recognize its association with abnormal cellular growth and its measurements are useful in monitoring these malignancies. Furthermore its interaction with LH and FSH places it within hormonal imbalances that can result in fertility issues.
