Description
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Cynomolgus monkey, Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.1 ng/mL,
Range:0.7 - 45 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate
Product details:
Human Albumin ELISA Kit ab179887 is a rapid single-wash 90-min ELISA kit to measure Human Albumin in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 0.1 ng/mL.
How the assay works
Human Albumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes native human Albumin protein (hsa) in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.
Human Albumin ELISA Kit ab179887 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Albumin ELISA kit ab179887
The Human Alb ELISA kit has been used in publications for the assessment of liver function in the construction of liver organoids or spheroids
(1)(2)
and for the assessment of Drug Induced Liver Toxicity (DILI)
(3)(4)
References:
Shuo Bai et al. 2023, PMID: 37199010,
Taniguchi H et al. 2023 PMID: 37108241,
Levner D et al. 2022, PMID: 36473994,
Ronxhi et al. 2022, PMID: 35790095
Save your precious samples
Our 384-well Human Albumin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Albumin Antibody Pair - BSA and Azide free
ab244051
- Anti-Albumin antibody [EPR20195-105] - BSA and Azide free (Capture)
ab244696
- Anti-Albumin antibody [EPR20195-16] - BSA and Azide free (Detector)
ab244962
Related and recommended products
Human Albumin ELISA kit ab179887 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Human ALT ELISA kit
ab234578
and Human AST ELISA Kit
ab263881
These ELISA kits are also available for Mouse/Rat:
- Mouse Albumin ELISA kit
ab207620
- Mouse ALT ELISA kit
ab282882
- Mouse AST ELISA kit
ab263882
- Rat Albumin ELISA kit
ab235642
- Rat ALT ELISA kit
ab234579
- Rat AST ELISA kit
ab263883
Kidney function marker assays include:
- Creatinine Assay Kit
ab65340
– suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
– suitable for use with serum
- Urea Assay Kit
ab83362
– suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin often referred to as serum albumin is a major protein in the bloodstream known for its role in maintaining osmotic pressure and transporting various molecules. It has a molecular mass of approximately 66.5 kDa. Synthesized in the liver albumin can be found throughout the body in the circulatory system. Besides its primary location in human plasma variants such as mouse serum albumin can be studied in animal models providing insight into albumin's function across species.
Biological function summary
Albumin carries out essential tasks like transporting substances such as hormones vitamins and drugs due to its high binding capacity. It serves as a vital protein for maintaining blood pH balance. Albumin does not function as part of a larger protein complex but acts independently to bind and transport various ligands thereby playing a role in removing free radicals and offering antioxidative protection.
Pathways
The transport and regulatory functions of albumin integrate into key biological systems like the Renin-Angiotensin System and drug metabolism pathways. Within these pathways albumin interacts with enzymes and other proteins facilitating drug distribution and metabolism. Its binding abilities allow it to modulate the bioavailability of active compounds acting in concert with proteins like globulins that also play roles in transporting biomolecules.
Low levels of albumin a condition known as hypoalbuminemia associate with liver diseases such as cirrhosis and chronic kidney disease impacting the body's ability to maintain fluid and electrolyte balance. Albumin's interaction with proteins like globulin can alter in these conditions affecting protein binding capacity and exacerbating disease symptoms. Tools like the albumin ELISA kit and albumin BCG assay facilitate the measurement of albumin levels in clinical diagnostics and research aiding in disease detection and monitoring.
