Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:< 50 µM,
Assay Platform:Microplate reader
Product details:
Tyrosine Assay Kit (Colorimetric) (ab185435) is a simple, yet sensitive assay that is able to detect normal and abnormal concentrations of Tyrosine in biological fluids. The assay is based on the enzymatic oxidation of Tyrosine producing a stable signal (OD 492 nm), which is directly proportional to the amount of Tyrosine. Sample preparation is minimal and does not require strenuous or complicated procedures. The assay can detect as low as 50 uM of Tyrosine in a variety of biological samples.
Other Notes
This product was previously called K573 Biovision Tyrosine Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Tyrosine (Tyr) is one of the four standard amino acids containing an aromatic group as a side chain. Its hydrophobicity is one of the main characteristics of this uncharged polar amino acid. In addition to being an essential amino acid Tyr is important in number of biological processes such as the synthesis of neurotransmitters, thyroid hormones, melanine, fumarate and acetoacetate. The pathology of abnormal concentrations of Tyr is well known in diseases including phenylketonuria, hypothyroidism, tyrosinemia, albinism, and alkaptonuria.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tyrosine also known as 4-hydroxyphenylalanine is an amino acid with a significant role in many biochemical processes. Tyrosine has a molecular mass around 181.19 g/mol. It is expressed in various tissues throughout the body including the brain muscles and skin. Tyrosine acts as a precursor for important neurotransmitters and hormones like dopamine norepinephrine and epinephrine playing mechanical roles in their synthesis.
Biological function summary
Tyrosine functions in protein synthesis as one of the 20 standard amino acids. It incorporates into proteins through ribosomal translation. Tyrosine is part of the large multi-enzyme complex involved in melanin production impacting pigmentation in skin and hair. This incorporation and synthesis contribute to tyrosine's varied biological roles including enzyme regulation.
Pathways
Tyrosine plays critical roles in several metabolic and signaling pathways. The catecholamine biosynthesis pathway utilizes tyrosine to synthesize neurotransmitters important for nervous system functions. Tyrosine kinase signaling pathways rely on tyrosine residues that undergo phosphorylation facilitating signal transduction for cellular processes. Proteins such as the insulin receptor and epidermal growth factor receptor are closely connected in these pathways.
Tyrosine imbalances can relate to conditions such as phenylketonuria (PKU) and alkaptonuria. PKU results from impaired conversion of phenylalanine to tyrosine affecting brain development and function. Alkaptonuria involves defective tyrosine metabolism leading to connective tissue and joint issues. Proteins like phenylalanine hydroxylase link to these disorders reflecting tyrosine's relevance in metabolic health.
