Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell culture supernatant, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 0.03 µM,
Range:0.0313 - 1 µM,
Assay time:2h,
Assay Platform:Microplate reader
Product details:
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. The traditional NAD/NADH and NADP/NADPH assays are based on monitoring the changes in NADH or NADPH absorption at 340 nm. The short UV wavelength of NAD/NADH and NADP/NADPH assays makes the traditional methods suffer low sensitivity and high interference.
Abcam's Colorimetric total NADP/NADPH Assay Kit (ab186033) provides a convenient method for detecting total NADP and NADPH. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction. There is no need to purify NADP/NADPH from the sample mix. The enzyme cycling reaction significantly increases detection sensitivity. The NADPH probe is a chromogenic sensor that has its maximum absorbance at 460 nm upon NADH reduction. The absorbance maximum increases to ~ 635 nm if the enhancer is added to the assay system. The absorption of the NADPH probe is directly proportional to the concentration of NADPH in the solution. The Colorimetric total NADP and NADPH Assay Kit provides a sensitive assay to detect as little as 0.03 μM total NADP/NADPH in a 100 μL assay volume.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
