Description
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 15 pg/mL,
Range:31.3 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate
Product details:
Human Betacellulin ELISA Kit (ab189575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Betacellulin protein in hep plasma, milk, serum, cit plasma, cell culture supernatant, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Betacellulin with 15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Betacellulin (BTC) is an EGF growth factor family member that binds to EGF Receptor family members. BTC is expressed as an 178 amino acid precursor form that is a single-pass transmembrane protein (this form is called probetacellulin). Subsequent cleavage produces the soluble and secreted 80 amino acid betacellulin fragment from the extracellular portion of probetacellulin. This assay reacts with the soluble betacellulin protein. There is high BTC protein sequence similarity among mammals.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Betacellulin (BTC) is a member of the EGF family of growth factors and is known for its role in cell development and proliferation. This glycoprotein with a molecular mass of approximately 32 kDa is derived from a transmembrane precursor and is cleaved to release the active form. Betacellulin is expressed prominently in the pancreas specifically in pancreatic beta cells and also observed in the gastric mucosa and kidneys. Due to its expression in these tissues it is vital in understanding cellular growth processes.
Biological function summary
Betacellulin stimulates the growth and differentiation of a variety of cell types strongly influencing the development of pancreatic beta cells and insulin-secreting cells. Betacellulin acts by binding to and activating the EGF receptor (EGFR) and ErbB-4 receptors that play a significant role in cellular signaling pathways. Betacellulin forms a part of the ErbB receptor signaling network interacting with other growth factors and receptors within the complex to mediate cellular responses.
Pathways
Betacellulin participates actively in the MAPK/ERK and PI3K/Akt signaling pathways. These pathways are important for regulating cell cycle progression apoptosis and cell survival. Betacellulin interacts with proteins like EGFR and ErbB-4 enhancing its effects on cell proliferation and tissue regeneration. By engaging in these signaling cascades betacellulin influences various downstream targets that impact cellular growth and differentiation processes.
Dysfunction in betacellulin signaling is implicated in diabetes and certain cancers. Reduced betacellulin activity is observed in diabetes where its role in promoting pancreatic beta-cell growth and insulin secretion becomes compromised. In cancer elevated levels of betacellulin are linked to the progression of tumors due to its role in cell proliferation. Within these disorders betacellulin's interactions with the EGFR and ErbB-4 play a significant role as these receptors are often overexpressed in tumorigenic processes further connecting betacellulin to the pathology of cancer development and progression.
