Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Milk, Serum, Tissue Culture Media,
Reacts with:Cow,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.3976 ng/mL,
Range:7.813 - 500 ng/mL,
Assay Platform:Microplate
Product details:
Abcam's IgG Cow ELISA kit (ab190517) is a two-site enzyme linked immunoassay (ELISA) for measuring IgG in Cow biological samples.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
