Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 14 pg/mL,
Range:14 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human BNP ELISA Kit ab193694 is a sandwich ELISA to measure Human BNP in serum, plasma, cell culture supernatant with a sensitivity of 14 pg/ml.
How the assay works
This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human BNP ELISA Kit ab193694 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
