Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Enzyme activity,
Assay Platform:Microplate reader
Product details:
Fumarase Activity Assay kit (ab196992) provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm.
The assay is simple and high-throughput ready and can detect less than 50 U/ml of fumarase activity in variety of sample types.
This product is manufactured by BioVision, an Abcam company and was previously called K596 Fumarase Activity Colorimetric Assay Kit. K596-100 is the same size as the 100 test size of ab196992.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Fumarase sometimes referred to as fumarate hydratase is an enzyme involved in the Krebs cycle. Its mechanical function involves the reversible hydration/dehydration of fumarate to malate. The enzyme has a molecular mass around 50 kilodaltons. Fumarase expression occurs in the mitochondria of eukaryotic cells and it also appears in the cytosol contributing to the compartmentalization of its enzymatic activity.
Biological function summary
Fumarase plays a critical role in cellular respiration by facilitating the conversion of fumarate to malate within the tricarboxylic acid (TCA) cycle. This enzyme does not operate as part of a larger protein complex but its activity is essential for maintaining the flow of carbon through the TCA cycle which is integral to energy production. Its activity is upregulated when cells require increased ATP generation.
Pathways
Fumarase participates prominently in the TCA cycle a vital pathway for aerobic respiration where it interconverts fumarate and malate. The enzyme supports cellular energy production and this is closely linked with other TCA cycle enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase which further facilitate efficient energy conversion.
Fumarase deficiency leads to severe neurological impairment often manifesting as encephalopathy. This deficiency occurs due to mutations in the FH gene which encodes for fumarase. Additionally a reduction in fumarase activity associates with fumarase-related hereditary leiomyomatosis and renal cell cancer (HLRCC) linking fumarase with altered metabolic states in tumorigenesis. Dysregulation within this context often involves interactions with metabolically similar enzymes complicating the metabolic landscape and contributing to oncogenic processes.
