Description
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Serum, Other biological fluids,
Assay type:Enzyme activity,
Sensitivity:= 0.06 mU/well,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Beta Lactamase Activity Assay Kit (Colorimetric) ab197008 is a simple and sensitive assay that can detect and quantify the enzymatic activity of beta lactamases, a large family of hydrolases.
The beta lactamase assay protocol is based on the hydrolysis of the substrate Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (detectable at OD=490 nm), which is directly proportional to the amount of beta-lactamase activity.
This product can detect as low as 0.06 mU of beta-lactamase activity from bacterial samples or from biological fluids infected with beta-lactamase expressing bacteria.
Beta lacatmase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode every 2-3 min for 30-60 min
This product is manufactured by BioVision, an Abcam company and was previously called K803 Beta-Lactamase Activity Colorimetric Assay Kit. K803-100 is the same size as the 100 test size of ab197008.
Beta Lactamases (β-Lactamases, βLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. βLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as β-lactams.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta lactamase sometimes referred to as penicillinase is an enzyme with a mass typically around 29 kDa. This enzyme hydrolyzes the beta-lactam ring an important structure in beta-lactam antibiotics which make this antibiotics ineffective. Beta lactamase is expressed in diverse bacterial species where its presence is a marker of antibiotic resistance. Scientists have developed inhibitors to block its action which are used in combination with antibiotics to combat resistant bacterial strains.
Biological function summary
Beta lactamase facilitates the survival of bacteria in the presence of beta-lactam antibiotics by breaking down these drugs. It is often part of a larger complex that includes various resistant genes enhancing its capacity to resist antibiotic pressures. The enzyme helps bacterial cells bypass the effects of these antibiotics by deactivating them before they can inhibit cell wall synthesis.
Pathways
Beta lactamase operates within the bacterial resistance mechanism directly impacting the beta-lactam antibiotic degradation pathway. This enzyme works alongside other resistance proteins like penicillin-binding proteins (PBPs) which are involved in cell wall biosynthesis. Their combined actions enable pathogenic bacteria to persist in environments with high antibiotic concentrations complicating disease treatment.
Beta lactamase has significant impact on bacterial infections such as those caused by Staphylococcus aureus and Escherichia coli which can lead to disorders like pneumonia and urinary tract infections. Its activity is linked to the failure of treatment with beta-lactam antibiotics. Related resistance proteins like metallo-beta-lactamases extend this resistance to even broader antibiotic classes presenting further challenges in managing these infections.
