Description
Key facts
Detection method:Colorimetric,
Sample types:Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay time:4h,
Assay Platform:Microplate reader
Product details:
MTS Assay Kit ab197010 uses a colorimetric method for the sensitive quantification of viable cells. It is based on a single ready-to-use MTS reagent. The MTS assay is used to assess cell proliferation, cell viability and cytotoxicity.
How the assay works
The MTS assay protocol is based on the reduction of the MTS tetrazolium compound by viable mammalian cells (and cells from other species) to generate a colored formazan dye that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye is quantified by measuring the absorbance at 490-500 nm. NB: MTS is also available as free molecule as
ab223881
(Tetrazolium inner salt cell proliferation reagent).
MTS assays are often used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. They are also used for the analysis of cytotoxic compounds like anticancer drugs and other toxic agents.
MTS assay protocol summary
- Add MTS reagent to cell culture media
- Incubate for 0.5 - 4 hours in standard culture conditions
- Shake plate briefly and measure absorbance at 490 nm.
The MTS assay is suitable for standard cell culture plates or 96-well and other microtitre well plates.
How other researchers are using MTS Assay Kit ab197010
The MTS assay kit has been used in publications with a variety of cell lines and primary cells, including: FB671 fibroblasts
, HUVEC and 4T1 cells
, IBRS-2 pig kidney cells
, mouse mesangial kidney cells
, human pancreatic ductal adenocarcinoma (PANC-1) and normal dermal fibroblast 3D co-cultures
, human primary fibroblasts
, human HuccT1 cholangiocarcinoma cells
, human glioma cell line
, human embryonic kidney cell line
, human 97L liver cancer cell line
, canine and human PBMCs
References: 1-Seminotti B et al 2019, 2-Amirshaghaghi A et al 2019, 3-Li SF et al 2019, 4-Widowati W et al 2018, 5-Betriu N and Semino CE 2018, 6-Leipnitz G et al 2018, 7-Lin HY et al 2018, 8-Nadaradjane A et al 2018, 9-Broguiere et al 2018, 10-Zhang K et al 2018, 11-Gardin C et al 2018
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
