Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts,
Reacts with:Mouse, Rat, Human,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate reader
Product details:
Abcam's Malate Dehydrogenase 1 (MDH1) Activity Assay kit is designed for the sensitive and accurate measurement of MDH1 activity in Human, mouse, and rat samples.
Malate Dehydrogenase 1 is the cytoplasmic isoform of the enzyme responsible for catalyzing the reversible oxidation of malate to oxaloacetate. The enzyme's activity requires the NAD+/NADH cofactor and participates primarily in the malate-aspartate shuttle.
The enzyme activity is determined by following the production of NADH in the following MDH1 catalyzed reaction:
Malate + NAD+ ↔ oxaloacetic acid + NADH
The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450nm (Dye molar extinction coefficient: 37000M
). In each well, only native MDH1 is immunocaptured from the sample; this removes all other enzymes, including MDH2 from the activity measurement.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cytosolic malate dehydrogenase commonly called MDH1 or malate dehydrogenase 1 is an enzyme that plays an important role in the citric acid cycle. Its main function is to catalyze the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. This enzyme has an approximate molecular mass of 36 kDa. MDH1 is expressed in the cytosol of various cell types throughout the body. It is critical in enabling cells to efficiently convert biochemical energy from nutrients into ATP which is vital for cellular function and survival.
Biological function summary
This enzyme supports numerous cellular processes by maintaining redox balance and facilitating energy production. MDH1 acts independently and isn't part of any larger protein complex. However its efficiency directly impacts the cycling of metabolites necessary for cellular respiration and energy metabolism. By converting malate into oxaloacetate MDH1 directly influences processes critical for the maintenance of cell health and function.
Pathways
MDH1 is an integral component of the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle it plays an important role in the conversion steps that drive energy generation within cells. Additionally the malate-aspartate shuttle is an important pathway connecting cytosolic and mitochondrial processes by facilitating the exchange of reducing equivalents. Through these pathways MDH1 interacts and collaborates with proteins like aspartate aminotransferase (GOT1) and mitochondrial malate dehydrogenase (MDH2) showcasing the interconnected nature of metabolic pathways.
Dysfunction or abnormal expression of MDH1 has been associated with various conditions including cancer and metabolic syndromes. In cancer aberrant MDH1 activity can lead to altered energy metabolism promoting tumor progression and survival. Additionally MDH1's role in glucose and lipid metabolism makes it relevant in metabolic syndrome where improper energy balance can exacerbate disease states. Proteins connected through these associations include hypoxia-inducible factor 1-alpha (HIF-1α) in cancer metabolism and insulin signaling proteins in metabolic syndromes further illustrating the broader implications of MDH1's function in health and disease.
