Description
Product details:
Protease Inhibitor Cocktail (EDTA-free) ab201111 inhibit a variety of endogenous proteases to protect your protein samples from degradation.
Ideal for mammalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, aspartic proteases
Inhibitors included - AEBSF hydrochloride, aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, pepstatin A
Supplied in DMSO (1 ml)
Preparation:
The recommended starting dilution is 1:200
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Individual components and concentrations of ab201111:
AEBSF hydrochloride:
serine protease inhibitor (100 mM)
Aprotinin (bovine lung):
serine protease inhibitor (0.08 mM)
Bestatin:
aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate:
serine/cysteine protease inhibitor (2 mM)
Pepstatin A:
aspartic protease inhibitor (1 mM)
This product is manufactured by BioVision, an Abcam company and was previously called K272 EZBlock™ Protease Inhibitor Cocktail, EDTA-Free. K272-1 is the same size as the 1 ml size of ab201111.
This product is manufactured by BioVision, an Abcam company and was previously called K272 EZBlock™ Protease Inhibitor Cocktail, EDTA-Free. K272-1 is the same size as the 1 ml size of ab201111.
Individual components and concentrations:
AEBSF hydrochloride:
serine protease inhibitor (100 mM)
Aprotinin (bovine lung):
serine protease inhibitor (0.08 mM)
Bestatin:
aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate:
serine/cysteine protease inhibitor (2 mM)
Pepstatin A:
aspartic protease inhibitor (1 mM)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
