Description
Key facts
Detection method:Colorimetric,
Sample types:Purified protein, Urine, Suspension cells, Adherent cells,
Assay type:Enzyme activity,
Sensitivity:< 0.001 mU/well,
Assay time:1h 10m,
Assay Platform:Microplate reader
Product details:
Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.
Urease assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- add additional reagents and incubate for 30 min
- analyze with microplate reader
Other Notes
This product was previously called K378 Biovision Urease Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil samples and fecal matter. Due to ammonia production, urease can play a role in the pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary tract pathogens.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Urease also known as urea amidohydrolase is an enzyme with a significant role in nitrogen metabolism. It mechanically catalyzes the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is commonly associated with a molecular mass ranging from 480 kDa to 600 kDa depending on species and isoform. Urease expresses predominantly in bacteria fungi and plants with significant incidence in gastrointestinal tract microorganisms like Helicobacter pylori. It is also present in some invertebrates but not usually in vertebrates.
Biological function summary
Urease plays a significant role in nitrogen metabolism by assisting the recycling of nitrogenous waste products and facilitating nutrient acquisition. It functions mainly as part of a multi-subunit complex usually composed of alpha beta and gamma subunits. These subunits coordinate to form a metalloenzyme that requires nickel ions to be catalytically active. This enzyme's ability to convert urea into ammonia makes it essential for nitrogen economy in various ecological settings.
Pathways
Urease is an integral component of the nitrogen cycle and is active in the urea degradation pathway. This pathway is important for recycling nitrogen in nature affecting soil fertility and plant growth. Urease links to proteins involved in ammonia assimilation such as glutamine synthetase which helps incorporate ammonia into amino acids. This relationship facilitates nitrogen availability for living organisms and enhances ecosystem productivity.
Urease relates to conditions like gastric ulcers and certain kidney stones. Helicobacter pylori employs urease to neutralize stomach acid enabling its survival and leading to gastritis and peptic ulcer disease. Also urease activity contributes to the formation of struvite stones in the urinary tract where urea-splitting bacteria like Proteus mirabilis produce ammonia leading to stone formation. These conditions illustrate urease's involvement with bacterial proteins like flagellar proteins and virulence factors that facilitate colonization and infection.
