Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Enzyme activity,
Assay time:45m,
Assay Platform:Microplate reader
Product details:
Cyclooxygenase COX Activity Assay Kit (Fluorometric) (ab204699) provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2 as well as other peroxidases, which may be present in the sample. Detection limit: 6 μU/mg.
How the Cox activity assay works
Cyclooxygenase (COX) Activity Assay Kit ab204699 measures COX peroxidase activity by detecting the conversion of arachidonic acid to prostaglandin G2 (PGG2), which reacts with a COX probe to produce a fluorescent signal proportional to enzyme activity.
Cyclooxygenase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- add arachidonic acid/NaOH solution
- analyze with microplate reader every 15 s for 30 min
This product is manufactured by BioVision, an Abcam company and was previously called K549 Cyclooxygenase (COX) Activity Assay Kit (Fluorometric). K549-100 is the same size as the 100 test size of ab204699.
Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by nonsteroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
