Description
Key facts
Detection method:Colorimetric,
Sample types:Inhibitor compounds,
Assay type:Enzyme activity,
Assay Platform:Microplate reader
Product details:
Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test to screen tyrosinase inhibitors.
Tyrosinase assay principle
In the tyrosinase assay, tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of kojic acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The absorbance values are used to calculate the tyrosinase activity of the samples
Tyrosinase Inhibitor assay protocol summary
- Screening compound preparation
- Preparation of controls
- Enzyme and substrate solution preparation
- Add enzyme solution and incubate at 25°C for 10 minutes
- Add substrate solution
- Measure absorbance (OD 510 nm) in a kinetic mode for 30-60 minutes at 25°C
How other researchers are using ab204715
Tyrosinase Inhibitor Assay Kit ab204715 has been used with a variety of sample types including:
- Escherichia coli Salmonella enterica and Pseudomonas aeruginosa
Highly ripe red guava extracts
Staphylococcus aureus
References: 1-Vilas-Boas A et al. 2023, 2-Bao S et al. 2023, 3-Oliveira A et al. 2022
Related and recommended products
To quantify Tyrosinase Activity, we recommend Tyrosinase Activity Assay Kit (Colorimetric)
ab252899
Other Notes
This product was previously called K575 Biovison Tyrosinase Inhibitor Screening Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tyrosinase also known as monophenol monooxygenase is a copper-containing enzyme with a mass of approximately 60-70 kDa. It catalyzes the oxidation of phenols such as tyrosine to quinones which are important precursors for melanin synthesis. Tyrosinase is predominantly expressed in melanocytes the cells responsible for pigment production in skin hair and eyes. Its expression also occurs in other tissues like the brain where it might play different roles.
Biological function summary
Within melanin production tyrosinase functions as the rate-limiting enzyme initiating the synthesis path by converting tyrosine into dopaquinone. This enzyme works as part of the melanogenic complex interacting with other enzymes such as tyrosinase-related proteins 1 and 2 to regulate melanin synthesis. The presence and activity of tyrosinase significantly impacts pigmentation processes and skin coloration.
Pathways
Relating tyrosinase to melanin biosynthetic and regulatory pathways reveal its central role. The enzyme is vital in the Raper-Mason pathway for melanin production where it interacts with proteins such as dopachrome tautomerase. It bridges the gap between external stimuli like UV radiation and cellular responses transforming them into pigmentation changes. The L-tyrosine and catecholamine biosynthesis pathways also influence tyrosinase activity linking it to broader metabolic processes.
Issues with tyrosinase can lead to conditions like albinism and melanoma. In oculocutaneous albinism type 1 (OCA1) a genetic mutation in the tyrosinase gene results in reduced or absent enzymatic activity causing hypopigmentation. In melanoma abnormal regulation of tyrosinase and its pathway associates with tumor progression and metastasis often in connection with factors such as microphthalmia-associated transcription factor (MITF) that modulate its expression.
