Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Quantitative,
Sensitivity:= 40 µM,
Assay time:30m,
Assay Platform:Microplate reader
Product details:
How the assay works
In Succinate Assay Kit protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
Succinate assay protocol summary:
- Add samples and standards to wells
- Add reaction mix and incubate for 30 min
- Analyze with a microplate reader
How other researchers are using
Succinate Assay Kit (Colorimetric)has been used in a variety of sample type including:
- Mouse brain
- IPSC-derived cardiomyocytes
- Human melanoma (C8161) cells
References:
1-Lancaster M et al. 2023
2-Srivastava S et al. 2023
3-Shen L et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.
Succinic acid (C
) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C
) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Succinic acid also known as butanedioic acid plays a significant mechanical role in cellular metabolism. It has a molecular mass of 118.09 g/mol and is important for both prokaryotic and eukaryotic cells. This compound is expressed in mitochondria where it acts as an intermediary in critical metabolic processes. The widespread presence across different types of organisms highlights its essential role in cellular energy production.
Biological function summary
Succinic acid contributes to energy production by participating in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. It is part of this complex cycle that produces ATP the energy currency of the cell. Succinic acid links different metabolic pathways facilitating the oxidation of nutrients to release energy. This has a broad impact on cellular function and organismal energy homeostasis emphasizing its indispensable role in general metabolism.
Pathways
Succinic acid is heavily involved in the TCA cycle and the electron transport chain. During the TCA cycle it transforms into fumarate through succinate dehydrogenase a vital process for energy extraction in cells. Succinic acid influences the electron transport chain where it facilitates ATP synthesis by interacting with complexes like Coenzyme Q10. These pathways are central to metabolic activity demonstrating how succinic acid integrates into the broader network of cellular respiration.
Succinic acid shows connections with metabolic disorders such as mitochondrial diseases and some cancers. Abnormal accumulation or defective metabolism of succinic acid can lead to mitochondrial dysfunction contributing to disorders like Leigh's syndrome. Additionally its involvement with the succinate dehydrogenase complex indicates potential links to various cancers given how mutations in this protein complex can lead to unregulated cell growth. These connections reflect the importance of succinic acid in maintaining metabolic stability highlighting its potential as a biomarker in disease contexts.
