Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue Lysate, Cell Lysate,
Sensitivity:= 1 µM,
Assay Platform:Microplate reader
Product details:
Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity 1 uM of F6P.
Other Notes
This product was previously called K689 Biovision PicoProbe Fructose-6-Phosphate Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Fructose-6-phosphate also known as F6P is an important intermediate in the glycolysis and gluconeogenesis pathways. It has a molecular mass of approximately 260.1 g/mol. This metabolite is primarily expressed in cellular cytoplasm where it is involved in biochemical transformations necessary for energy production. Although it does not have specified protein complex associations fructose-6-phosphate remains an integral compound in metabolic processes.
Biological function summary
Fructose-6-phosphate plays a significant role in carbohydrate metabolism. It serves as a substrate for numerous reactions including the conversion from glucose-6-phosphate to fructose-6-phosphate an important step catalyzed by the enzyme phosphoglucose isomerase. Fructose-6-phosphate's involvement in these reactions makes it essential for balancing energy through anabolic and catabolic activities.
Pathways
Fructose-6-phosphate is highly involved in glycolysis and gluconeogenesis. In glycolysis it is further converted to fructose-16-bisphosphate a reaction facilitated by phosphofructokinase-1 (PFK-1) an enzyme that plays a critical role in regulating the pathway. Additionally in gluconeogenesis fructose-6-phosphate converts back to glucose-6-phosphate functioning in reverse to produce glucose from non-carbohydrate precursors.
Alterations in the metabolism of fructose-6-phosphate have been linked to diabetes and cancer. In diabetes abnormal regulation of glycolysis and gluconeogenesis can affect blood sugar levels where proteins like PFK-1 and phosphoglucose isomerase may play a part. Understanding these connections provides insight into developing therapies for managing metabolic disorders.
