Description
Key facts
Detection method:Fluorescent,
Sample types:Purified protein, Tissue Lysate, Plasma, Cell Lysate,
Assay type:Enzyme activity,
Assay time:20m,
Assay Platform:Microplate reader
Product details:
Lipoprotein Lipase Assay Kit (Fluorometric) ab204721 is a simple and accurate assay for measuring Lipoprotein Lipase (LPL) activity.
How the assay works
Lipoprotein Lipase Assay Kit (Fluorometric) contains a quenched substrate that fluoresces upon hydrolysis by lipoprotein lipase (LPL). The fluorometric intensity is directly proportional to the amount of substrate hydrolyzed.
This lipase assay detects total lipase activity when no inhibitor is used. Comparing results in the presence or absence of an LPL inhibitor allows for quantification of LPL activity specifically.
Our results indicate that the majority (~90%) of lipase activity detected by this kit in post-heparin treated mouse plasma is from LPL. To determine the exact LPL specific activity in mouse plasma, measure activity in pre- and post-heparin treated plasma.
Lipoprotein Lipase assay summary:
- Add samples and standards to wells
- Add reaction mix
- Pre-incubate the plate at 37°C for 10 minutes
- Measure fluorescence (Ex/Em= 482/515 nm) in a kinetic mode
for 1 hour at 37°C
How other researchers are using ab204721
Lipoprotein Lipase Assay Kit (Fluorometric) has been used in a variety of sample type including:
- Human Caco-2 cells
- Mouse Adipose tissue homogenate
- Mouse spleen samples
References: 1-Pang G al. 2022, 2-Gencer et al. 2021, 3-Ringel A et al. 2020
Other Notes
This product was previously called K721 Lipoprotein Lipase Activity Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Lipoprotein lipase (LPL) is a member of the lipase family that hydrolyzes triglycerides in chylomicrons and very low-density lipoprotein (VLDL). Digestion of triglycerides in VLDL by LPL leads to their conversion to intermediate-density lipoprotein (IDL) and then low-density lipoprotein (LDL). LPL is found attached to the luminal surface of endothelial cells in the heart, muscle, and adipose tissue. Mutations in lipoprotein lipase can lead to a variety of disorders such as lipoprotein metabolism, hypertriglyceridemia etc. Overexpression of LPL in mice has been shown to promote obesity and insulin resistance.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
