Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Enzyme activity,
Assay Platform:Microplate reader
Product details:
Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at OD=515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.
Other Notes
This product was previously called K675 Biovision Sulfatase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sulfatase enzymes also known by names such as arylsulfatase facilitate the hydrolysis of sulfate esters into alcohols and sulfate ions. These enzymes typically have varied molecular masses generally ranging from 45 to 65 kDa. Sulfatases are expressed in many tissues but show higher concentrations in the liver kidneys and gonads. They function both in the lysosomes and on extracellular surfaces highlighting their versatility in both intracellular and extracellular environments.
Biological function summary
Sulfatases participate in essential cellular processes such as hormone regulation cell signaling and the degradation of glycosaminoglycans. The enzymes exist as a part of a larger group of proteins linked to the metabolism of sulfated compounds. These functions make them indispensable for numerous physiological activities including modification and breakdown of sulfate group-bearing molecules. Sulfatases have a critical role in maintaining homeostasis by altering the charge and solubility of biologically essential compounds.
Pathways
Sulfatases integrate into key biological pathways like the lysosomal degradation pathway and the steroid biosynthesis pathway. In lysosomal degradation sulfatases help break down complex carbohydrates that are otherwise difficult to process. In steroid biosynthesis they modify steroid sulfates back into active steroids influencing hormonal balance. The pathway involves other proteins such as estrogen sulfotransferases which work collaboratively with sulfatases to regulate hormonal actions.
Sulfatase deficiencies are linked to various metabolic and genetic disorders including multiple sulfatase deficiency and mucopolysaccharidosis. Without proper function of these enzymes individuals can experience an accumulation of sulfated molecules which causes significant cellular dysfunction. Disorders can also involve proteins like the iduronate-2-sulfatase where mutations inhibit enzyme function leading to conditions such as Hunter syndrome. Both conditions highlight the need for functional sulfatase activity for normal physiological health.
