Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Assay type:Competitive,
Sensitivity:= 2.84 pg/mL,
Range:4.1 - 1000 pg/mL,
Assay Platform:Microplate
Product details:
Abcam's Arg
-Vasopressin
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Arg
-Vasopressin in tissue culture media.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a Biotin conjugated- Arg
-Vasopressin antigen and a polyclonal rabbit antibody specific to Arg
-Vasopressin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Arg8-Vasopressin captured in the plate.
Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei. It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions.
AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Arg8-Vasopressin also known as antidiuretic hormone (ADH) or AVP is a peptide hormone with a molecular mass of around 1 kDa. It is synthesized in the hypothalamus and secreted by the posterior pituitary gland. Arg8-Vasopressin exerts its effects mainly through binding to receptors on the kidney collecting ducts. This hormone plays a direct mechanical role in the reabsorption of water by increasing the permeability of kidney tubules to water. Elevated levels of Arg8-Vasopressin can lead to water retention influencing osmoregulation in the body.
Biological function summary
Arg8-Vasopressin plays a significant role in maintaining fluid balance and blood pressure homeostasis. It is not part of a larger protein complex but interacts with various cellular pathways through its receptors namely V1a V1b and V2. The hormone facilitates vasoconstriction via V1 receptors therefore impacting cardiovascular function. Additionally it modulates central nervous system activities influencing behavioral and social responses. Arg8-Vasopressin impacts adrenal corticotropic hormone secretion through its effect on the anterior pituitary.
Pathways
Arg8-Vasopressin is integrated into the renin-angiotensin-aldosterone system a critical pathway for blood pressure and blood volume regulation. This hormone interacts with the angiotensin II peptide facilitating aldosterone release and further promoting sodium uptake in kidneys. It also is part of the hypothalamic-pituitary-adrenal axis modulating stress response through feedback loops. Connections to oxytocin are present as both peptides share structural similarities and can affect one another's receptor pathways.
Arg8-Vasopressin is important in the context of diabetes insipidus and hyponatremia. In diabetes insipidus reduced action or secretion of the hormone results in excessive urine production and dehydration. Hyponatremia can occur when excess Arg8-Vasopressin leads to water retention and electrolyte imbalance. A link exists between Arg8-Vasopressin and oxytocin in conditions involving psychological stress as both influence emotional behavior and stress resilience. Understanding these connections aids in therapeutic targeting and the development of receptor-specific treatments.
