Description
Key facts
Detection method:Colorimetric,
Sample types:Purified protein, Tissue Lysate, Cell Lysate,
Assay Platform:Microplate reader
Product details:
Abcam's BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.
The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu
), which is generated by reduction of cupric cation (Cu
) by the protein in alkaline conditions. The Cu
-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 μg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.
Support on how to determine protein concentration can be found
This product is manufactured by BioVision, an Abcam company and was previously called K814 Extra Sense™ BCA Protein Assay Kit. K814-2500 is the same size as the 2500 test size of ab207002.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
