Description
Key facts
Detection method:Colorimetric,
Reacts with:Mouse,
Assay type:ELISA set,
Sensitivity:= 46.52 pg/mL,
Range:125 - 8000 pg/mL,
Assay Platform:Reagents
Product details:
Mouse Albumin Matched Antibody Pair Kit is an ELISA development kit to quantify Mouse Albumin by sandwich ELISA method with a sensitivity of 46.52 pg/mL.
How the Assay Works
A capture antibody specific of the antigen to be measured is coated on a 96-well plate. Samples are added to the wells followed by a biotin-labelled antibody. After elimination of the unbound antigen and detection antibody, a strepatavidin-HRP solution is added to the wells, followed by a TMB substrate. HRP catalyzes the oxidation of TMB in the presence of hydrogen peroxide, producing a blue-colored product. After the reaction is stopped, the solution turns yellow. The intensity of the yellow color, read at 450nm is directly proportional to the amount of antigen present in the sample.
Assay specificity
The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Albumin. Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
protocol summary
1. Coat the 96-well plate with the capture antibody overnight at 4C or 2 hours at room temperature on a plate shaker
2. Wash and block to reduce non specific binfing.
3. Add standards and samples to appropiate wells and incubate at room temperature.
4. Wash
5. Add the biotin-labelled detector antibody to all wells and incubate at room temperature
6. Wash
7. Add the HRP-Streptavidin solution and incubate at room temperature
8. Wash
9. Add the TMB substrate solution and incubate
10. Add stop solution and read at 450 nm
Complete your experiment with:
Matched Antibody Pair Kit ELISA Accessory Pack (10 x 96-well plates) (ab210905): contains 10 96-well plates, coating and blocking buffers, wash buffer, TMB substrate, stop solution and streptavidin-HRP solution.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
