Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Urine, Plasma, Serum, Cell Lysate,
Assay type:Quantitative,
Sensitivity:= 10 µM,
Assay Platform:Microplate reader
Product details:
D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG).
How the assay works
In the D 2 Hydroxyglutarate assay, D-2-Hydroxyglutarate is oxidized to alpha-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples.
D 2 Hydroxyglutarate assay protocol summary
- Add samples and standards to wells
- Add reaction mix
- Incubate for 1 hour at 37°C
- Analyze with a microplate reader
How other researchers are using ab211070
D 2 Hydroxyglutarate Assay Kit (Colorimetric) has been used in a variety of sample types including:
- Human glioma samples
- Primary human astrocytes
- Human Diffuse large B cell lymphoma
References: 1-Chen X al. 2023, 2-Bredel M et al. 2023, 3-Cai Y et al. 2023
Other Notes
This product was previously called K213 Biovision D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and alpha-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of alpha ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
